The South American tomato pinworm, Tuta absoluta, is considered worldwide as one of the most important tomato pests. Insecticide resistance reported on the moth and the awareness about the negative impact of agrochemicals on the environment and human health have raised the demand for new control tools. Among these, RNA interference (RNAi) can represent a valid tool to be included into new control strategies against this pest. Here we report the results of trials aimed at evaluating the effects of dsRNAs targeting acetylcholinesterase (AChE), nicotinic acetylcholine alpha 6 (nAChRs), ryanodine (RyRs) receptors by injection and root delivery. In the injection procedure, 2 and 5 µg of dsRNA were able to reduce the gene expression in a range of 62.7–75.4%, inducing a maximum mortality rate of 92.59%. The dsRNAs administered at 5 µg by root absorption revealed the potential of this delivery system to affect the gene expression (47–69% reduction) and the mortality (ranging from 67.1 to 80.5%) of treated specimens. The delivered dsRNAs (both injected and root administered) affected the weight of both T. absoluta pre-pupae and pupae. The selected AChE, nAChRs and RyRs genes can be suitable targets for T. absoluta control by means of in planta delivery dsRNAs. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

RNAi in Tuta absoluta management: effects of injection and root delivery of dsRNAs

Campolo O;PALMERI, Vincenzo
2019-01-01

Abstract

The South American tomato pinworm, Tuta absoluta, is considered worldwide as one of the most important tomato pests. Insecticide resistance reported on the moth and the awareness about the negative impact of agrochemicals on the environment and human health have raised the demand for new control tools. Among these, RNA interference (RNAi) can represent a valid tool to be included into new control strategies against this pest. Here we report the results of trials aimed at evaluating the effects of dsRNAs targeting acetylcholinesterase (AChE), nicotinic acetylcholine alpha 6 (nAChRs), ryanodine (RyRs) receptors by injection and root delivery. In the injection procedure, 2 and 5 µg of dsRNA were able to reduce the gene expression in a range of 62.7–75.4%, inducing a maximum mortality rate of 92.59%. The dsRNAs administered at 5 µg by root absorption revealed the potential of this delivery system to affect the gene expression (47–69% reduction) and the mortality (ranging from 67.1 to 80.5%) of treated specimens. The delivered dsRNAs (both injected and root administered) affected the weight of both T. absoluta pre-pupae and pupae. The selected AChE, nAChRs and RyRs genes can be suitable targets for T. absoluta control by means of in planta delivery dsRNAs. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
2019
RNA interference Lepidoptera Ryanodine receptor Acetylcholinesterase Nicotinic acetylcholine receptor Tomato
File in questo prodotto:
File Dimensione Formato  
Majidiani_2019_PestSci_RNAi_editor.pdf

non disponibili

Tipologia: Versione Editoriale (PDF)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 894.22 kB
Formato Adobe PDF
894.22 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Majidiani _2019_J Pest Sci_ RNAi_post(1324).pdf

Open Access dal 26/02/2020

Tipologia: Documento in Post-print
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 326.5 kB
Formato Adobe PDF
326.5 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12318/1324
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 29
  • ???jsp.display-item.citation.isi??? 28
social impact