Spatial and temporal fluctuations in nitrate (NO3 -) availability are very common in agricultural soils. Therefore, understanding the molecular and physiological mechanisms involved in regulating NO3 - uptake in regions along the primary root is important for improving the NO3 - uptake efficiency (NUpE) in crops. Different regions of maize primary root, named R1, R2 and R3, NO3 - starved for 3 days, were exposed to 50 μM NO3 -. Electrophysiological measurements (membrane potential and H+ and NO3 - fluxes) and NPF6.3, NRT2.1, NAR2.1, MHA1, MHA3 and MHA4 gene expression analyses were carried out. The results confirmed variable spatial and temporal patterns in both NO3 - and H+ fluxes and gene expression along the primary maize root. A significant correlation (P = 0.0023) between nitrate influx and gene transcript levels was observed only when NAR2.1 and NRT2.1 co-expression were considered together, showing for the first time the NRT2.1/NAR2.1 functional interaction in nitrate uptake along the root axis.Taken together these results suggest differing roles among the primary root regions, in which the apical part seem to be involved to sensing and signaling in contrast with the basal root which appears to be implicate in nitrate acquisition.

NAR2.1/NRT2.1 functional interaction with NO3- and H+ fluxes in high-affinity nitrate transport in maize root regions

Lupini A;Araniti F;Sunseri F;Abenavoli MR
2016-01-01

Abstract

Spatial and temporal fluctuations in nitrate (NO3 -) availability are very common in agricultural soils. Therefore, understanding the molecular and physiological mechanisms involved in regulating NO3 - uptake in regions along the primary root is important for improving the NO3 - uptake efficiency (NUpE) in crops. Different regions of maize primary root, named R1, R2 and R3, NO3 - starved for 3 days, were exposed to 50 μM NO3 -. Electrophysiological measurements (membrane potential and H+ and NO3 - fluxes) and NPF6.3, NRT2.1, NAR2.1, MHA1, MHA3 and MHA4 gene expression analyses were carried out. The results confirmed variable spatial and temporal patterns in both NO3 - and H+ fluxes and gene expression along the primary maize root. A significant correlation (P = 0.0023) between nitrate influx and gene transcript levels was observed only when NAR2.1 and NRT2.1 co-expression were considered together, showing for the first time the NRT2.1/NAR2.1 functional interaction in nitrate uptake along the root axis.Taken together these results suggest differing roles among the primary root regions, in which the apical part seem to be involved to sensing and signaling in contrast with the basal root which appears to be implicate in nitrate acquisition.
2016
Ion-selective microelectrode; Maize root regions; NRT2.1
File in questo prodotto:
File Dimensione Formato  
Lupini_2016_PPB_NAR_editor.pdf

non disponibili

Descrizione: versione dell'Editore
Tipologia: Versione Editoriale (PDF)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.23 MB
Formato Adobe PDF
1.23 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Lupini_2016_PPB_NAR_postprint(1).pdf

Open Access dal 01/01/2019

Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 698 kB
Formato Adobe PDF
698 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12318/797
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 33
social impact