The aerial part of Artemisia arborescens L. (Asteraceae) was extracted with water and methanol, and both extracts were fractionated using n-hexane, chloroform, ethyl acetate and n-butanol. The potential phytotoxicity of both crude extracts and their fractions were assayed in vitro on seed germination and root growth of lettuce (Lactuca sativa L.), a sensitive species largely employed in the allelopathy studies. The inhibitory activities were analysed by dose-response curves and the ED 50 were estimated. Crude extracts strongly inhibited both germination and root growth processes. The fraction-bioassay indicated the following hierarchy of phytotoxicity for both physiological processes: ethyl acetate ≥ n-hexane > chloroform ≥ n-butanol. On the n-hexane fraction, GC-MS analyses were carried out to characterise and quantify some of the potential allelochemicals. Twenty-one compounds were identified and three of them, camphor, trans-caryophyllene and pulegone were quantified.
Allelopathic potential of Artemisia arborescens: Isolation, identification and quantification of phytotoxic compounds through fractionation-guided bioassays / Araniti, F.; Lupini, A.; Sorgona', A.; Conforti, F; Marrelli, M.; Statti, G. A.; Menichini, F; Abenavoli, M. R.. - In: NATURAL PRODUCT RESEARCH. - ISSN 1478-6419. - 27:10(2013), pp. 880-887. [10.1080/14786419.2012.691491]
Allelopathic potential of Artemisia arborescens: Isolation, identification and quantification of phytotoxic compounds through fractionation-guided bioassays
Araniti F.;Lupini A.;SORGONA' A.;Abenavoli M. R.
2013-01-01
Abstract
The aerial part of Artemisia arborescens L. (Asteraceae) was extracted with water and methanol, and both extracts were fractionated using n-hexane, chloroform, ethyl acetate and n-butanol. The potential phytotoxicity of both crude extracts and their fractions were assayed in vitro on seed germination and root growth of lettuce (Lactuca sativa L.), a sensitive species largely employed in the allelopathy studies. The inhibitory activities were analysed by dose-response curves and the ED 50 were estimated. Crude extracts strongly inhibited both germination and root growth processes. The fraction-bioassay indicated the following hierarchy of phytotoxicity for both physiological processes: ethyl acetate ≥ n-hexane > chloroform ≥ n-butanol. On the n-hexane fraction, GC-MS analyses were carried out to characterise and quantify some of the potential allelochemicals. Twenty-one compounds were identified and three of them, camphor, trans-caryophyllene and pulegone were quantified.File | Dimensione | Formato | |
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