An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to identify the composition of fungal communities associated with different strawberry organs (leaves,flowers,immature and mature fruits),grown on a farm using management practices that entailed the routine use of various chemical pesticides. ITS2 sequences clustered into 316 OTUs and Ascomycota was the dominant phyla(95.6%)followed by Basidiomycota (3.9%). Strawberry plants supported a high diversity of microbial organisms,but two genera, Botrytis and Cladosporium, were the most abundant,representing 70–99% of the relative abundance(RA)of all detected sequences. According to alpha and beta diversity analyses, strawberry organsdisplayedsignificantlydifferentfungalcommunitieswithleaveshaving the most diverse fungal community,followed by flowers,andfruit.Theinterruptionofchemicaltreatmentsforonemonthresultedinasignificantmodificationinthestructureofthe fungal community of leaves and flowers while immature and mature fruit were not significantly affected.Several plant pathogens of other plant species,that would not be intuitively expected to be present on strawberry plants such as Erysiphe,were detected,while some common strawberry pathogens,such as Rhizoctonia,were less evident or absent.

Metagenomic analysis of fungal diversity on strawberry plants and the effect of management practices on the fungal community structure of aerial organs

Li Destri Nicosia MG;Schena L.
2016

Abstract

An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to identify the composition of fungal communities associated with different strawberry organs (leaves,flowers,immature and mature fruits),grown on a farm using management practices that entailed the routine use of various chemical pesticides. ITS2 sequences clustered into 316 OTUs and Ascomycota was the dominant phyla(95.6%)followed by Basidiomycota (3.9%). Strawberry plants supported a high diversity of microbial organisms,but two genera, Botrytis and Cladosporium, were the most abundant,representing 70–99% of the relative abundance(RA)of all detected sequences. According to alpha and beta diversity analyses, strawberry organsdisplayedsignificantlydifferentfungalcommunitieswithleaveshaving the most diverse fungal community,followed by flowers,andfruit.Theinterruptionofchemicaltreatmentsforonemonthresultedinasignificantmodificationinthestructureofthe fungal community of leaves and flowers while immature and mature fruit were not significantly affected.Several plant pathogens of other plant species,that would not be intuitively expected to be present on strawberry plants such as Erysiphe,were detected,while some common strawberry pathogens,such as Rhizoctonia,were less evident or absent.
File in questo prodotto:
File Dimensione Formato  
Abdelfattah_2016_Plos One_Metagenomic_editor.PDF

accesso aperto

Descrizione: Versione editoriale - OPEN ACCESS
Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 2.69 MB
Formato Adobe PDF
2.69 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12318/6054
Citazioni
  • ???jsp.display-item.citation.pmc??? 25
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 57
social impact