Nero Siciliano is an autochthonous pig breed that is reared mainly in semi-extensive systems in northeastern Sicily.Despite its economic importance and well-appreciated meat products, this breed is currently endangered. Consequently,an analysis of intra-breed variability is a fundamental step in preserving this genetic resource and its breedingsystem. In this work, we used 25 microsatellite markers to examine the genetic composition of 147 unrelatedNero Siciliano pigs. The total number of alleles detected (249, 9.96 per locus) and the expected heterozygosity(0.708) indicated that this breed had a high level of genetic variability. Bayesian cluster analysis showed that themost likely number of groups into which the sample could be partitioned was nine. Based on the proportion of eachindividuals genome derived from ancestry, pigs with at least 70% of their genome belonging to one cluster were assignedto that cluster. The cluster size ranged from 7 to 17 (n = 108). Genetic variability in this sub-population wasslightly lower than in the whole sample, genetic differentiation among clusters was moderate (FST 0.125) and the FISvalue was 0.011. NeighborNet and correspondence analysis revealed two clusters as the most divergent. Molecularcoancestry analysis confirmed the good within-breed variability and highlighted the clusters that retained the highestgenetic diversity.Key words: cluster analysis, coancestry, intra-breed genetic variation, microsatellites, Nero Siciliano pig breed.
|Titolo:||Molecular characterization and genetic structure of the Nero Siciliano pig breed|
|Data di pubblicazione:||2010|
|Appare nelle tipologie:||1.1 Articolo in rivista|