Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation

A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions wasvalidated for ten Phytophthora species with a broad host range from forest trees to crop species. The melting curveanalysis of the amplicons specifically grouped all species into 10 respective unique and distinct HRM curve profiles.The analysis of the normalised HRM melting curves, assigning P. nicotianae as a normalised reference genotype,revealed that the genotype similarities among all the species were adequately low, indicating that Ypt1 marker wassufficient to identify and differentiate the tested species. This HRM method is rapid and reproducible allowing theidentification of Phytophthora species and the screening of eventual variants eliminating the separate steps and reducingthe risk of contamination.