To improve the study of mixed microalgal populations, three naturally evolved marinemicroalgal cultures were subjected to a light crushing mechanical treatment using a siliconspatula coupled with zymolyase treatment at four concentrations: 5, 10, 20 and 25 U/ml, for15, 30, 45 and 60 min before being observed under a microscope. The enzyme concentrationof 20 U/ml after 45 min reduces the size of macroscopic microalgal aggregates and improvesthe microscopic observation of the different microalgal species comprising the population.There was no improvement using the higher enzyme concentration. This paper proposes anew approach to the study of naturally evolved microalgal populations which is useful fordistinguishing the morphology of the different species present in the population andallowing for the identification by classical keys, and also to obtain a pure culture from aninoculum of mixed species by using a micromanipulator for cell counting.

A simple and rapid method for separation and isolation of marine algal species from naturally evolved populations.

SIDARI, Rossana
2016

Abstract

To improve the study of mixed microalgal populations, three naturally evolved marinemicroalgal cultures were subjected to a light crushing mechanical treatment using a siliconspatula coupled with zymolyase treatment at four concentrations: 5, 10, 20 and 25 U/ml, for15, 30, 45 and 60 min before being observed under a microscope. The enzyme concentrationof 20 U/ml after 45 min reduces the size of macroscopic microalgal aggregates and improvesthe microscopic observation of the different microalgal species comprising the population.There was no improvement using the higher enzyme concentration. This paper proposes anew approach to the study of naturally evolved microalgal populations which is useful fordistinguishing the morphology of the different species present in the population andallowing for the identification by classical keys, and also to obtain a pure culture from aninoculum of mixed species by using a micromanipulator for cell counting.
Mixed microalgal populations; natural evolution; zymolyase; mechanical treatment; algal EPS
File in questo prodotto:
File Dimensione Formato  
Sidari_2016_MarineBiolRes_A simple_post.pdf

embargo fino al 01/03/2017

Descrizione: Post-print
Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 1.12 MB
Formato Adobe PDF
1.12 MB Adobe PDF Visualizza/Apri
Sidari_2016_MarineBiolRes_A simple_editor.pdf

non disponibili

Descrizione: Versione editoriale
Tipologia: Versione Editoriale (PDF)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.89 MB
Formato Adobe PDF
2.89 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12318/1385
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact