IntroductionClonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone “Lempso” is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling.To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method.Extracts were subjected to solid phase extraction and the qualitative/quantitative profile was elucidated through ultra-high performance liquid chromatography (UHPLC) coupled to photodiode array (PDA) and ion trap time-of-flight (IT-TOF) mass spectrometry detection, and compared to both Cleopatra mandarin (Citrus reticulata) and blood orange (Citrus sinensis (L.) Osbeck) Sanguinello varieties. The antioxidant activity was assessed by pre-column 2,2′-diphenyl-1-picrylhydrazyl (DPPH) reaction coupled to UHPLC-PDA.Lempso is characterised by flavonoids (17) and anthocyanins (8). Flavanones content (Hesperidin: 57.19 ± 0.49, Vicenin-2: 4.59 ± 0.03, Narirutin: 5.78 ± 0.13 mg/100 mL) was considerably higher than Cleopatra and Sanguinello varieties. The developed DPPH-UHPLC-PDA method provides information regarding the single contributions to antioxidant activity, highlighting how Ferulic acid, Quercetin and Cyanidin derivatives possess considerable radical scavenging activity (> 50%). The total antioxidant activity was also evaluated and compared with positive controls, showing higher scavenging activity than Cleopatra and Sanguinello (IC50: 333.76 ± 10.81 μg/mL vs. 452.62 ± 10.81 and 568.39 ± 26.98 μg/mL, respectively).These data evidence the nutraceutical potential of Lempso variety, which could be an ingredient for functional beverages.
Flavonoid Composition of Tarocco (Citrus sinensis L. Osbeck) Clone "Lempso" and Fast Antioxidant Activity Screening by DPPH-UHPLC-PDA-IT-TOF / Sommella, E; Pagano, F; Pepe, G; Ostacolo, C; Manfra, M; Chieppa, M; DI SANZO, Rosa; Carabetta, S; Campiglia, P; Russo, Mariateresa. - In: PHYTOCHEMICAL ANALYSIS (ONLINE). - ISSN 1099-1565. - 28:6(2017), pp. 521-528. [10.1002/pca.2701]
Flavonoid Composition of Tarocco (Citrus sinensis L. Osbeck) Clone "Lempso" and Fast Antioxidant Activity Screening by DPPH-UHPLC-PDA-IT-TOF.
Di Sanzo Rosa;Russo Mariateresa
2017-01-01
Abstract
IntroductionClonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. Within Citrus sinensis varieties, Tarocco clone “Lempso” is a typical product of the Calabria region (Italy) characterised by its red pulp. This is the first report concerning its accurate profiling.To characterise in detail the flavonoid composition of Lempso clone and to compare its antioxidant potential with other Citrus varieties by a fast screening method.Extracts were subjected to solid phase extraction and the qualitative/quantitative profile was elucidated through ultra-high performance liquid chromatography (UHPLC) coupled to photodiode array (PDA) and ion trap time-of-flight (IT-TOF) mass spectrometry detection, and compared to both Cleopatra mandarin (Citrus reticulata) and blood orange (Citrus sinensis (L.) Osbeck) Sanguinello varieties. The antioxidant activity was assessed by pre-column 2,2′-diphenyl-1-picrylhydrazyl (DPPH) reaction coupled to UHPLC-PDA.Lempso is characterised by flavonoids (17) and anthocyanins (8). Flavanones content (Hesperidin: 57.19 ± 0.49, Vicenin-2: 4.59 ± 0.03, Narirutin: 5.78 ± 0.13 mg/100 mL) was considerably higher than Cleopatra and Sanguinello varieties. The developed DPPH-UHPLC-PDA method provides information regarding the single contributions to antioxidant activity, highlighting how Ferulic acid, Quercetin and Cyanidin derivatives possess considerable radical scavenging activity (> 50%). The total antioxidant activity was also evaluated and compared with positive controls, showing higher scavenging activity than Cleopatra and Sanguinello (IC50: 333.76 ± 10.81 μg/mL vs. 452.62 ± 10.81 and 568.39 ± 26.98 μg/mL, respectively).These data evidence the nutraceutical potential of Lempso variety, which could be an ingredient for functional beverages.File | Dimensione | Formato | |
---|---|---|---|
Sommella2017_PA_Flavonoid_postprint.pdf
Open Access dal 08/06/2018
Descrizione: Post print autore
Tipologia:
Documento in Post-print
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
1.18 MB
Formato
Adobe PDF
|
1.18 MB | Adobe PDF | Visualizza/Apri |
Sommella2017_PA_Flavonoid_editorial.pdf
non disponibili
Tipologia:
Versione Editoriale (PDF)
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
722.65 kB
Formato
Adobe PDF
|
722.65 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.