This study aimed to evaluate the inheritance of the trait ochratoxin A adsorption in two wine strains of Saccharomyces cerevisiae and their 46 descendants. Each strain was inoculated in triplicate in test tubes containing 10 ml of must obtained from the Calabrian Zibibbo white grape variety, artifi cially contaminated with ochratoxin A to reach a total content of 4.10 ng/ml. The microvinifi cation trials were performed at 25°C. After 30 days, ochratoxin A values ranged from 0.74 to 3.18 ng/ml, from 0.01 to 2.69 ng/ml, and from 0.60 to 2.95 ng/ml respectively in wines, in lees after washing, and in the saline solution used to wash the lees. The analysis of OTA in wines was performed to fi nd the residual toxin content after yeast activity, thus obtaining technological evidence of yeast infl uence on wine detoxifi cation. The analysis of OTA in lees after washing was performed to distinguish the OTA linked to cells. The analysis of OTA in the saline solution used to wash the lees was performed to distinguish the OTA adsorbed on yeast cell walls and removed by washing, thus focusing on the adsorption activity of wine yeast through electrostatic and ionic interactions between parietal mannoproteins and OTA. Ploidy of the two parental strains was controlled by fl ow cytometry. Results demonstrated that the ochratoxin A adsorption is genetically controlled and is a polygenic inheritable trait of wine yeasts. The majority of the descendants are characterized by a great and signifi cant diversity compared to their parents. Both the parental strains had genome sizes consistent with their being diploid, so validating the observed results. These fi ndings constitute an initial step to demonstrate the mechanisms of inheritance and establish breeding strategies to improve the ochratoxin A adsorption trait in wine yeasts. This will allow a decrease in the ochratoxin A content of contaminated musts during winemaking, by using genetically improved wine yeasts.
|Titolo:||Ochratoxin A adsorption phenotype: An inheritable yeast trait|
CARIDI, Andrea Domenico Maria F. A. (Corresponding)
|Data di pubblicazione:||2012|
|Appare nelle tipologie:||1.1 Articolo in rivista|