The study was carried out using 212 glucose-fermenting yeasts isolated from Calabrian and Sicilian samples of must and wine. They were screened for β-glucosidase activity in Petri paltes containing arbutin agar medium. Eleven strains, (three apiculate yeasts and eight elliptic yeasts) were able to perform arbutin hydrolysis, but only strain C5, identified as Pichia anomala, maintained the ability to hydrolyse arbutin even in presence of remarkable amounts of glucose or ethanol. This strain did not show the capacity to excrete the β-glucosidase into the medium; effectively, the enzymatic activity in the culture supernatant was near to zero. However, its intracellular activity was high.

Screening for yeasts able to hydrolyse arbutin in the presence of glucose or ethanol

CARIDI A.;SIDARI R.
2005

Abstract

The study was carried out using 212 glucose-fermenting yeasts isolated from Calabrian and Sicilian samples of must and wine. They were screened for β-glucosidase activity in Petri paltes containing arbutin agar medium. Eleven strains, (three apiculate yeasts and eight elliptic yeasts) were able to perform arbutin hydrolysis, but only strain C5, identified as Pichia anomala, maintained the ability to hydrolyse arbutin even in presence of remarkable amounts of glucose or ethanol. This strain did not show the capacity to excrete the β-glucosidase into the medium; effectively, the enzymatic activity in the culture supernatant was near to zero. However, its intracellular activity was high.
File in questo prodotto:
File Dimensione Formato  
CARIDI_2005_AM_SCREENING_editor.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 81.6 kB
Formato Adobe PDF
81.6 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.12318/2516
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact